Cryptococcus gattii is the main pathogen of cryptococcosis in healthy patients and is treated
mainly with fluconazole and amphotericin B. The combination of these drugs has been
questioned because the mechanisms of action could lead to a theoretical antagonistic
interaction. We evaluated distinct parameters involved in the in vitro combination of
fluconazole and amphotericin B against Cryptococcus gattii. Fourteen strains of C. gattii
were used for the determination of MIC, fractional inhibitory concentration, time-kill curve …

MATERIALS AND METHODS C. gattii strains. We tested 14 strains of C. gattii (nine clinical
and three environmental isolates, all from the culture collection of the Laboratório de
Micologia da Universidade Federal de Minas Gerais, Minas Gerais, Brazil, and two
reference strains from the culture collection of the University of Georgia, Atlanta, GA). All of
the isolates were maintained on slant Sabouraud dextrose agar (SDA) at 4 C. Inoculum
preparation. Prior to the tests, the strains were subcultured on SDA for 48 h at 35 C. The …