The purpose of paper analyzes influence of foundation mass on dynamic response of beam on non-uniform foundation subjected to a moving vehicle. This foundation model includes non-uniform linear elastic springs, shear layer, viscous damping and special consideration of the influence of a characteristic parameter of foundation mass. The moving vehicle is assumed to be consisted of two nodal masses that are connected by means of a spring-damper components. The equation of motion for the beam-vehicle-foundation interaction element is derived by means of dynamic balance principle. After by assembling the stiffness, damping and mass matrices, and vectors of nodal loads of all elements based on finite element method, the governing equations of motion for the integrated system are obtained and solved by step-by-step integration method procedure. The accuracy of the algorithm is verified by comparing the numerical results with the other numerical results in the literature. Also, the effects of characteristic parameter of foundation mass on dynamic analysis of beamvehicles interaction are investigated detail. The results show that the influence of foundation mass has effects significantly on dynamic response of beam-vehicle interaction and more increasing dynamic response than others without influence of foundation mass.