The diacylglycerol kinases (DGK) regulate diacylglycerol-based signals by phosphorylating
this key lipid intermediate to phosphatidic acid. Here, we have investigated the spatial and
temporal regulation of diacylglycerol kinase ζ (DGKζ) in living Jurkat T-cells expressing a
muscarinic type I receptor. Using real time confocal videomicroscopy, we show the rapid
translocation of a green fluorescent protein-tagged enzyme from the cytosol to the plasma
membrane following receptor stimulation. The generation of a panel of truncations …