The effect of administering acetaminophen and energy drink on serum proteins during sub-chronic alcohol consumption was investigated on 42 Wister albino rats that were divided into seven groups of six rats each. Group 1 served as the normal control and received 1ml of bottled water, group two received alcohol (2.5 ml/kg body weight), group three was given energy drink (5ml/kg body weight), group four received paracetamol (28.55 mg/kg body weight), group five received same dose of alcohol and energy drink, group six received same dose of alcohol and paracetamol, and group seven received same dose of alcohol, energy drink and paracetamol. The administration was carried out twice daily for 14 days. From the result, there were nonsignificant increases (p> 0.05) in serum total protein (g/dl) of the groups treated with alcohol (59.75±1.93), energy drink (62.20±2.03), alcohol+ energy drink (60.40±3.33) and alcohol+ energy drink+ paracetamol (61.20±1.85) compared to the control group (58.67±1.36) and non-significant decreases (p> 0.05) in the groups receiving paracetamol (58.00±1.34) and alcohol+ paracetamol (57.00±1.29). Serum albumin levels (g/dl) were significantly decreased (p< 0.05) in groups treated with energy drink (19.20±0.20), alcohol+ energy drink (19.80±0.85) and alcohol+ paracetamol (22.25±0.25) compared to the control group (25.33±0.21). Serum globulin levels (g/dl) were significantly increased (p< 0.05) in groups treated with energy drink (43.00±2.02) and alcohol+ energy drink (40.60±3.36) compared to the control group (33.33±1.38). The results show that sub-chronic consumption of alcohol alone or in combination with energy drink and paracetamol does not alter serum protein levels adversely since values obtained were within reference ranges.