In Madhya Pradesh 20-40% of the, black soils are deficient in sulphur (Shinde et al. 1982). The sulphur application in these soils increases not only crop yield but also improves crop quality (Saraf 1988) through higher N-fIXation in legumes (Tandon 1991). The requirement of P and S in legumes and oilseeds is generally higher than in cereals (Shinde and Soni 1981) and response ofS and its utilization is related to P (Shinde et al. 1982). Besides, S has ameliorating effect on saline and alkaline soils, resulting in healthy rhizosphere, which increases the activity of micro-symbiont on roots of host plants (Tandon 1991), though the response to inoculation depends on climate, temperature, soil type and nutrient supply etc, Since information is meagre on the effect of different levels of sulphur applied through different sources on symbiotic and biometrical traits of soybean [Glycine max (L.) Merr.], an experiment was conducted on these aspects. The field experiment was conducted at Sehore during the rainy season (kharif) 1990. The soil was Vertisol havingpH 7.5, electrical conductivity 0.57 dS/m, with available N, P, K and S 185 kg/ha, 9 kg/ba, 380 kg/ha and 12