An experiment was conducted at Instructional farm, Department of Horticulture, College of Agriculture, Ummedganj, Kota (Agriculture University, Kota). The experiment was laid out in a Factorial Completely Randomized Design with 32 treatment combinations and these treatments were replicated thrice. The treatments comprised of 8 pre-sowing seed treatments (T) viz., T0-Control (Storage without treatment), T1-Lime juice treatment, T2-KNO3 at 1%, T3-KNO3 at 2%, T4-KNO3 at 3%, T5-Thiourea at 1%, T6-Thiourea at 1.5% and T7-Thiourea at 2% and four Storage periods (S) viz., S0-sowing 0 days after seed extraction, S1-sowing 7 days after seed extraction, S2-sowing 14 days after seed extraction and S3-sowing 21 days after seed extraction. Irrespective of the chemical treatments Sowing of seed immediately after extraction (fresh seed) observed maximum nitrogen (1.70%) content, Phosphorus (0.38%) content, Potassium (0.96%) content, chlorophyll a (1.34 mg/g), chlorophyll b (0.38 mg/g), total chlorophyll (1.73 mg/g) and maximum relative water content (87.14%) in leaf as well. However seed treatment T4 KNO3@ 3% recorded maximum phosphorus (0.39%), potassium (1.02%), chlorophyll a (1.40%), chlorophyll b (0.38%), total chlorophyll (1.78%) and relative water content (86.50%) in leaves at 60 DAS. Maximum nitrogen content (1.79%) showed that the treatment T7 thiourea@ 2%. Treatment combination S0T4 recorded maximum phosphorus content (0.42%), maximum Potassium content (1.05%) and chlorophyll b (0.43 mg/g) content at 60 DAS in leaves of acid lime.