Effect of the in vitro culture system on the kinetics of blastocyst development and sex ratio of bovine embryos
Bovine blastocysts were produced using 6 different systems: 5 commonly used in vitro
culture systems (synthetic oviduct fluid medium-SOF-without fetal calf serum, SOF
supplemented with 10% serum for the entire culture period, SOF supplemented with 10%
serum from Day 4 of culture, M199 coculture with bovine oviduct epithelial cells, M199
coculture with granulosa cell monolayer) and 1 in vivo culture system involving collection of
blastocysts from superovulated bovine donors at Day 7. Zygotes obtained from IVM/IVF were …
culture systems (synthetic oviduct fluid medium-SOF-without fetal calf serum, SOF
supplemented with 10% serum for the entire culture period, SOF supplemented with 10%
serum from Day 4 of culture, M199 coculture with bovine oviduct epithelial cells, M199
coculture with granulosa cell monolayer) and 1 in vivo culture system involving collection of
blastocysts from superovulated bovine donors at Day 7. Zygotes obtained from IVM/IVF were …
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