Stripe rust (yellow rust) caused by Puccinia striiformis f. sp. tritici, is a serious disease of wheat occurs with cool and moist weather conditions during the growing season. Investigations on influence of weather conditions were conducted on wheat cultivar PBW 343 during 2012-13 and 2013-14 under natural conditions and under artificial (inoculated) conditions. During 2012-13 and 2013-14 there was higher disease incidence and severity but during 2013-14 rate of disease incidence and severity was slightly less as compared to 2012-13 in both the experiments. It was observed that the prevailing weather conditions during both the years influenced disease progress. During both the years maximum temperature, minimum temperature and sunshine hours were significantly positively correlated, whereas morning and evening relative humidity were negatively correlated with disease severity and incidence. During 2012-13 disease incidence and severity was comparatively more which might be due to more rainfall during the crop season. Analysis of different weather parameters with stripe rust under natural and artificial conditions indicated that temperature and relative humidity were most important weather parameters which effected disease incidence and severity maximum and could be used as key indicators in stripe rust forewarning under Punjab conditions.