AlxMoNbTaTiV alloys are designed according to the binary phase diagrams and calculation of phase diagrams (CALPHAD). The phase formation and elemental segregation are predicted using the CALPHAD method. The effects of Al on microstructures, and mechnical properties of AlxMoNbTaTiV are investigated, which verified the results of calculated dendritic microstructure and BCC structure. The strengthening mechanism is proved as the result of chemical and electrical interactions. The serration behavior in the compression test at 25 °C, 500 °C, 700 °C, 900 °C is discussed. At room temperature, addition of Al enhanced the dynamic aging effects in low concentration. The effects became weaker with the increase of Al content. At 500 °C, higher Al concentration leads to stronger serrated flow.