In this research, the rate of shoot proliferation from different nodes in different positions on shoot [uppermost node (N₁), the second node (N₂) and the third node (N₃)] from two mature guava cultivars, white fleshed Local-1 and red fleshed Local-2 on different culture media (MS and WPM) with different BA concentrations was investigated. The results showed that in both cultivars, the best BA concentration was 1.5 mg L^-1. There was a significant interaction between bud positions, culture media and cultivars on shoot proliferation and shoot length. In Local-1 maximum shoot number (2.86) was recorded from buds in N₂ position on MS medium followed by 2.43 shoots/explant from buds in N₂ positions on WPM medium. In Local-2 on both medium the buds in N₁ position had significantly a higher number of shoots/explant (1.57 on MS and 1.86 on WPM) than the buds in N₃ positions (0.43 on MS and 0.71 on WPM). Shoot length, had a similar trend.