Efficient CRISPR/Cas9-mediated genome editing using a chimeric single-guide RNA molecule
The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted
mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous
delivery of a DNA template for homology-directed repair (HDR). Here, we used
CRISPR/Cas9 to generate targeted double-strand breaks and to deliver an RNA repair
template for HDR in rice (Oryza sativa). We used chimeric single-guide RNA (cgRNA)
molecules carrying both sequences for target site specificity (to generate the double-strand …
mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous
delivery of a DNA template for homology-directed repair (HDR). Here, we used
CRISPR/Cas9 to generate targeted double-strand breaks and to deliver an RNA repair
template for HDR in rice (Oryza sativa). We used chimeric single-guide RNA (cgRNA)
molecules carrying both sequences for target site specificity (to generate the double-strand …
Efficient CRISPR/Cas9-mediated genome editing using a chimeric single-guide RNA molecule.
HB Haroon Butt, AE Ayman Eid, ZA Zahir Ali, MAM Atia… - 2017 - cabidigitallibrary.org
The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted
mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous
delivery of a DNA template for homology-directed repair (HDR). Here, we used
CRISPR/Cas9 to generate targeted double-strand breaks and to deliver an RNA repair
template for HDR in rice (Oryza sativa). We used chimeric single-guide RNA (cgRNA)
molecules carrying both sequences for target site specificity (to generate the double-strand …
mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous
delivery of a DNA template for homology-directed repair (HDR). Here, we used
CRISPR/Cas9 to generate targeted double-strand breaks and to deliver an RNA repair
template for HDR in rice (Oryza sativa). We used chimeric single-guide RNA (cgRNA)
molecules carrying both sequences for target site specificity (to generate the double-strand …
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