The presence of agricultural effluents in water poses significant threats, adversely impacting both human health and the ecosystem. To address these challenges, this study employs a multifaceted approach involving the incorporation of a novel chitosan/MgO/Zeolite adsorbent and the utilization of an isolated fungal strain, Aspergillus fumigatus. The adsorbent properties were carefully characterized using X-ray diffraction, scanning electron microscopy, and Fourier transform infrared spectroscopy. The optimization results indicated that the maximum adsorption 86.1% for malathion was observed with CS/MgO/Zeolite after 40 min, dosage 0.4 g/l, malathion concentration 10mgL⁻¹ and pH(7). The adsorption capacity of CS/MgO/Zeolite for malathion, as determined by the Langmuir isotherm, is reported to be 83.3 mgg⁻¹. The Freundlich isotherm and the pseudo-second-order kinetic model were applied to the adsorption data, resulting in an impressive R² value of 0.99.The results from desorption and regeneration investigations demonstrate that the CS/MgO/Zeolite composite remains effective for up to five cycles.