Efficient virus-mediated genome editing in plants using the CRISPR/Cas9 system

Z Ali, A Abul-Faraj, L Li, N Ghosh, M Piatek, A Mahjoub… - Molecular plant, 2015 - cell.com
… of mutant plants from the seed progeny. The virus-mediated genome editing platform we …
viruses could also be used to deliver the gRNA molecules to various plant species. Our …

Virus-mediated genome editing in plants using the CRISPR/Cas9 system

A Mahas, Z Ali, M Tashkandi, MM Mahfouz - Plant Genome Editing with …, 2019 - Springer
… , the efficient delivery of genome engineering reagents into plant cells is crucial for the effective
use … Thus, our work expands the utility of the CRISPR/Cas9 system for functional genomic

Efficient virus-mediated genome editing in cotton using the CRISPR/Cas9 system

J Lei, Y Li, P Dai, C Liu, Y Zhao, Y You, Y Qu… - Frontiers in Plant …, 2022 - frontiersin.org
Plant virus-mediated sgRNA delivery and expression have great advantages; sgRNA … ,
resulting in efficient gene editing efficiency. In this study, a VIGE system based on cotton leaf …

Pea early-browning virus-mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis

Z Ali, A Eid, S Ali, MM Mahfouz - Virus research, 2018 - Elsevier
efficient sgRNA delivery tools for CRISPR/Cas9-based genome engineering in model and
non-model plant … Indeed, more 200 plant species are natural hosts for TRV, and this increases …

Virus-induced plant genome editing

Y Oh, H Kim, SG Kim - Current opinion in plant biology, 2021 - Elsevier
… virus-induced genome editing (VIGE) technology in plants: (1) … , and (3) how to increase the
efficiency with which viruses are … plant virus vectors can be used efficiently in plant-genome

Efficient genome editing in cotton using the virus-mediated CRISPR/Cas9 and grafting system

WF Guo, DD Guo, F Li, SZ Shang, TW Li, YC Tang… - Plant Cell Reports, 2023 - Springer
… Here, we report the virus-mediated genome editing in cotton in a fraction of the time and
minimal workload that would take by stably transforming the entire Cas9/sgRNA cassette. The …

A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing

K Yin, T Han, G Liu, T Chen, Y Wang, AYL Yu, Y Liu - Scientific reports, 2015 - nature.com
Virus-mediated gene knockout will not have these limitations. Here, we report that Cabbage
… This may explain why our VIGE showed very high efficiency of genome editing in this study. …

Challenges facing CRISPR/Cas9-based genome editing in plants

S Son, SR Park - Frontiers in plant science, 2022 - frontiersin.org
… increase the efficiency of genome editing in these plant organelles. … of edited plants
without the stable integration of a transgene. Several CRISPR/Cas9 systems allow gene editing

Virus-induced gene editing and its applications in plants

C Zhang, S Liu, X Li, R Zhang, J Li - International Journal of Molecular …, 2022 - mdpi.com
… for CRISPR/Cas9 cassette delivery and genome editing. We … The CRISPR/Cas9 system
can be used to create insertions … gene regulation systems in plants include the virus-mediated

Potato virus X vector-mediated DNA-free genome editing in plants

H Ariga, S Toki, K Ishibashi - Plant and Cell Physiology, 2020 - academic.oup.com
… Here, we describe a method for highly efficient targeted mutagenesis in Nicotiana … Pea
early-browning virus-mediated genome editing via the CRISPR/Cas9 system in Nicotiana …