In this study, a new potentiometric sensor selective to copper (II) ions was developed and characterized. The developed sensor has a polymeric membrane and contains 4.0% electroactive material (ionophore), 33.0% poly (vinyl chloride)(PVC), 63.0% bis (2-ethylhexyl) sebacate (BEHS) and 1.0% potassium tetrakis (p-chlorophenyl) borate (KTpClPB). This novel copper (II)-selective sensor exhibits a Nernstian response over a wide concentration range from 1.0× 10-6 to 1.0× 10-1 mol L-1 with a slope of 29.6 (±1.2) mV decade-1, and a lower detection limit of 8.75× 10-7 mol L-1. The sensor, which was produced economically by synthesizing the ionophore in the laboratory, has a good selectivity and repeatability, fast response time and stable potentiometric behaviour. The potential response of the sensor remains unaffected of pH in the range of 5.0-10.0. Based on the analytical applications of the sensor, we showed that it can be used as an indicator electrode in the quantification of Cu 2+ ions by potentiometric titration against EDTA, and can also be successfully utilized for the determination of copper (II) ions in different real samples.