Using organic nanofibers as dispensers for pheromones and kairomones in plant protection for disrupting insect chemical communication is a novel approach aiming at popularizing this technique in organic and integrated plant production. Expected advantages of the nanofibers are highly controlled spatiotemporal release rates of pheromones/kairomones, improved climatic stability, and mechanized application. Dispenser types used so far show deficiencies in one or more of these requirements. Mechanical application of pheromone dispensers is a new approach to reduce the costs of manual labour. Therefore the environmentally compatible, highly specific and efficient technique of mating disruption may become an alternative to the use of synthetic pesticides in integrated pest management. The electrospun nanofibers are highly elastic, which prevents break-off of smaller pieces, and polymers used are biocompatible. Due to the tiny scale of nanofibers the mass input both for pheromones and for polymeric nanofibers is extremely low. Major environmental benefits are high control specificity, very low concentrations of residues and minimal risk towards development of resistant insect strains. We tested organic polymer fibers, made from the biodegradable polymer Ecoflex®(BASF), treated with Lobesia botrana (Lepidoptera: Tortricidae) sex pheromone,(E, Z)-7, 9-dodecadienyl acetate, in cage tests placed inside vineyards. With this experimental setup it is possible to show the applicability of mating disruption dispensers for the use in plant protection against insect pests in vineyards. We used Isonet LE dispensers (Shin-Etsu) as a positive control. The Ecoflex® nanofiber-pheromone-dispensers show a mating disruption effect which is comparable to the efficacy of the Isonet LE dispensers for at least three weeks. After that, the mating disruption effect observed tapers off to a level where it is insufficient for plant protection purposes. But, in principle, our first prototype of a nanofiber pheromone dispenser has proved its efficacy for mating disruption purposes. Modifications of the fibers, currently under development, open up the possibility of extending the disruption effect to a period of several months’ duration, enough to cover the entire flight period of Lobesia.