Cross priming amplification: mechanism and optimization for isothermal DNA amplification

G Xu, L Hu, H Zhong, H Wang, S Yusa, TC Weiss… - Scientific reports, 2012 - nature.com
… : polymerase chain reaction 1 and isothermal amplification. The polymerase chain reaction
… or more cross primers with a 3′ end complementary to the target template sequence and a …

Construction of tandem repeats of DNA fragments by a polymerase chain reaction-based method

JR Chen, ZN Deng, YB Chen, BW Hu, JJ Lü… - DNA and Cell …, 2012 - liebertpub.com
… We describe a new application of megaprimer polymerase chain reaction (PCR) for …
The reverse primer had a higher T m , a 3′ end complementary to the DRE sequence and …

Quadruple-analyte chemiluminometric hybridization assay. Application to double quantitative competitive polymerase chain reaction

DS Elenis, PC Ioannou, TK Christopoulos - Analytical chemistry, 2007 - ACS Publications
… The IVR-IS probe comprises a segment at the 3‘end complementary to IVR-IS and a
sequence at the 5‘ end complementary to the oligonucleotide that is conjugated to β-galactosidase. …

Competitive enzymatic reaction to control allele-specific extensions

E Hultin, M Kaller, A Ahmadian… - Nucleic acids …, 2005 - academic.oup.com
… of the fact that DNA polymerase extends 3′-termini mismatches with slower reaction kinetics.
The use of Proteinase K allows genotyping at elevated reaction temperature as compared …

A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn …

RMK Dale, BA McClure, JP Houchins - Plasmid, 1985 - Elsevier
… Incubate the reaction at 37C for x minutes (approximately 40-… Heat the T4 DNA polymerase
reaction for 10 min at 65C to … 3’ end and a 5’ end complementary to the oligomer. Because of …

Primer design strategy for denaturation bubble-mediated strand exchange amplification

J Deng, Y Li, W Shi, R Liu, C Ma, C Shi - Analytical biochemistry, 2020 - Elsevier
… of polymerase is also obviously affected by reaction temperature [15,16]. Thus, the optimization
of reaction … self-complementary and 3′ end complementary of the primers would also …

A novel electrochemical biosensor based on dynamic polymerase-extending hybridization for E. coli O157: H7 DNA detection

L Wang, Q Liu, Z Hu, Y Zhang, C Wu, M Yang, P Wang - Talanta, 2009 - Elsevier
… An original and dynamic polymerase-extending (PE) DNA … of Taq DNA polymerase and
dNTPs under controlled reaction … A 120-base ss-DNA with 3′ end complementary sequence …

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides

MG Mohsen, D Ji, ET Kool - Nucleic acids research, 2019 - academic.oup.com
… While thermal stability is a benefit, attaching these types of linkers could impede polymerase
reaction rates while the alkyl group itself does not contribute to the polymerase reaction itself…

Terminal redundancy and the origin of replication of Rous sarcoma virus RNA.

JM Coffin, WA Haseltine - Proceedings of the National …, 1977 - National Acad Sciences
… ' end complementarypolymerase reactions of RSV virions to determine what portions of
the genome are complementary to it. The products of an endogenous DNA polymerase reaction

Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene

ZA Shabarova, IN Merenkova… - Nucleic acids …, 1991 - academic.oup.com
… the polymerase reaction in the … polymerase I (Klenow fragment) or reverse transcriptase,
the 30S initiator complexes are capable of inhibiting the reactions involving T4 DNA polymerase