JR Chen, ZN Deng, YB Chen, BW Hu, JJ Lü… - DNA and Cell …, 2012 - liebertpub.com
… We describe a new application of megaprimer polymerase chain reaction (PCR) for … The reverse primer had a higher T m , a 3′ endcomplementary to the DRE sequence and …
… The IVR-IS probe comprises a segment at the 3‘endcomplementary to IVR-IS and a sequence at the 5‘ endcomplementary to the oligonucleotide that is conjugated to β-galactosidase. …
E Hultin, M Kaller, A Ahmadian… - Nucleic acids …, 2005 - academic.oup.com
… of the fact that DNA polymerase extends 3′-termini mismatches with slower reaction kinetics. The use of Proteinase K allows genotyping at elevated reaction temperature as compared …
… Incubate the reaction at 37C for x minutes (approximately 40-… Heat the T4 DNA polymerase reaction for 10 min at 65C to … 3’ end and a 5’ endcomplementary to the oligomer. Because of …
J Deng, Y Li, W Shi, R Liu, C Ma, C Shi - Analytical biochemistry, 2020 - Elsevier
… of polymerase is also obviously affected by reaction temperature [15,16]. Thus, the optimization of reaction … self-complementary and 3′ endcomplementary of the primers would also …
… An original and dynamic polymerase-extending (PE) DNA … of Taq DNA polymerase and dNTPs under controlled reaction … A 120-base ss-DNA with 3′ endcomplementary sequence …
… While thermal stability is a benefit, attaching these types of linkers could impede polymerase reaction rates while the alkyl group itself does not contribute to the polymerasereaction itself…
JM Coffin, WA Haseltine - Proceedings of the National …, 1977 - National Acad Sciences
… ' endcomplementary … polymerasereactions of RSV virions to determine what portions of the genome are complementary to it. The products of an endogenous DNA polymerasereaction …
ZA Shabarova, IN Merenkova… - Nucleic acids …, 1991 - academic.oup.com
… the polymerasereaction in the … polymerase I (Klenow fragment) or reverse transcriptase, the 30S initiator complexes are capable of inhibiting the reactions involving T4 DNA polymerase …