Engineering bromodomains with a photoactive amino acid by engaging 'Privileged'tRNA synthetases
S Wagner, B Sudhamalla, P Mannes… - Chemical …, 2020 - pubs.rsc.org
Chemical Communications, 2020•pubs.rsc.org
Site-specific placement of unnatural amino acids, particularly those responsive to light, offers
an elegant approach to control protein function and capture their fleeting 'interactome'.
Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized
photo-crosslinker, by introducing several key features including easy synthetic access, site-
specific incorporation by 'privileged'synthetases and superior crosslinking efficiency, to
develop photo-crosslinkable bromodomains suitable for 'interactome'profiling.
an elegant approach to control protein function and capture their fleeting 'interactome'.
Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized
photo-crosslinker, by introducing several key features including easy synthetic access, site-
specific incorporation by 'privileged'synthetases and superior crosslinking efficiency, to
develop photo-crosslinkable bromodomains suitable for 'interactome'profiling.
Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting ‘interactome’. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by ‘privileged’ synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for ‘interactome’ profiling.
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