In the study, the silver nanoparticle were grafted on graphene oxide with and without use of Allium sativum as stabilizing or reducing agent and ultrasonic assistant have been successfully prepared and then their comparative anti-angiogenesis and hemolytic studies have been carried out. The synthesized particles have been characterized by using modern techniques including P-XRD, AT-FTIR, UV-Visible spectroscopy, SEM analysis, and BT-90 Nano size analyzer (BT-90). The results revealed uniform distribution of Ag on the GO sheet in both cases with mean particle size of 32.96 nm with d-spacing 2.3634 nm and 11.65 nm with d-spacing 2.3594 nm for GO-Ag-G NPs and GO-Ag NPs, respectively. The maximum absorbance was obtained at 448.08 nm of GO-Ag-G NPs and 443.29 of GO-Ag NPs. Both types of NPs showed dose-dependent anti-angiogenesis activity analyzed via CAM assay and showed% hemolysis of range (0.0971-. 1246) for GO-Ag-G NPs and (0.14-0.5797) for GO-Ag NPs.