produce high-quality protein. The optimal conditions for D. salina to co-produce intracellular
pools of both compounds, however, are yet unknown. This study investigated a two-phase
cultivation strategy to optimize combined high-quality protein and carotenoid production of
D. salina. In phase-one, a gradient of nitrogen concentrations was tested. In phase-two,
effects of nitrogen pulse and high illumination were tested. Results reveal optimized protein …