Enzymatic synthesis of 2′-ara and 2′-deoxy analogues of c-di-GMP
AS Shchokolova, AN Rymko, SV Kvach… - … and Nucleic Acids, 2015 - Taylor & Francis
AS Shchokolova, AN Rymko, SV Kvach, PS Shabunya, SA Fatykhava, AI Zinchenko
Nucleosides, Nucleotides and Nucleic Acids, 2015•Taylor & FrancisThe substrate specificity of recombinant full-length diguanylate cyclase (DGC) of
Thermotoga maritima with mutant allosteric site was investigated. It has been originally
shown that the enzyme could use GTP closest analogues–2′-deoxyguanosine-5′-
triphosphate (dGTP) and 9-β-D-arabinofuranosyl-guanine-5′-triphosphate (araGTP) as the
substrates. The first demonstrations of an enzymatic synthesis of bis-(3′-5′)-cyclic dimeric
deoxyguanosine monophosphate (c-di-dGMP) and the previously unknown bis-(3′-5′) …
Thermotoga maritima with mutant allosteric site was investigated. It has been originally
shown that the enzyme could use GTP closest analogues–2′-deoxyguanosine-5′-
triphosphate (dGTP) and 9-β-D-arabinofuranosyl-guanine-5′-triphosphate (araGTP) as the
substrates. The first demonstrations of an enzymatic synthesis of bis-(3′-5′)-cyclic dimeric
deoxyguanosine monophosphate (c-di-dGMP) and the previously unknown bis-(3′-5′) …
The substrate specificity of recombinant full-length diguanylate cyclase (DGC) of Thermotoga maritima with mutant allosteric site was investigated. It has been originally shown that the enzyme could use GTP closest analogues – 2′-deoxyguanosine-5′-triphosphate (dGTP) and 9-β-D-arabinofuranosyl-guanine-5′-triphosphate (araGTP) as the substrates. The first demonstrations of an enzymatic synthesis of bis-(3′-5′)-cyclic dimeric deoxyguanosine monophosphate (c-di-dGMP) and the previously unknown bis-(3′-5′)-cyclic dimeric araguanosine monophosphate (c-di-araGMP) using DGC of T. maritima in the form of inclusion bodies have been provided.
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