Lymnaeid snails transmit medical and veterinary trematodiases, mainly fascioliasis. Vector specificity of fasciolid parasites defines disease distribution and characteristics. Control of fascioliasis is to de-link the life cycle of fluke, by destroying the Lymnaeid snails. Different Bait formulations were fed to snail Lymnaea acuminata in clear glass aquaria having diameter of 30 cm. Snail attractant containing bait formulations were prepared from different binary combination of amino acid (valine, aspartic acid, lysine and alanine 10 mM) in 100 ml of 2% agar solution+ sub-lethal (20% and 60% of 24h LC50 and 96h LC50) doses of different molluscicides (eugenol, ferulic acid, umbelliferone, and limonene). Snails were fed bait with sub-lethal concentration of different molluscicides inside the snail attractant pellets, which caused a significant inhibition in alkaline phosphatase (ALP) and acetylcholinesterase (AChE) activity in the nervous tissue of the vector snail L. acuminata. Combination of different amino acids such as valine+ aspartic acid, lysine+ valine, lysine+ alanine and alanine+ valine was used as attractant with active molluscicidal components. Maximum inhibition in ALP (23.57% of control) and AChE (49.48% of control) activity were observed in the nervous tissue of the L. acuminata exposed to 60% of 96h LC50 of ferulic acid, umbelliferone, respectively in the bait pellets containing valine+ aspartic acid as attractant.