Equilibrium between monomeric and dimeric mitochondrial F1–inhibitor protein complexes

L Domı́nguez-Ramı́rez, G Mendoza-Hernandez… - FEBS …, 2001 - Wiley Online Library
Mg‐ATP particles from bovine heart mitochondria have more than 95% of their F1 in
complex with the inhibitor protein (IF1). The F1–IF1 complex was solubilized and purified.
The question addressed was if this naturally occurring complex existed as monomers or
dimers. Size exclusion chromatography and electron microscopy showed that most of the
purified F1–IF1 complex was a dimer of two F1–IF1. As determined by the former method,
the relative concentrations of dimeric and monomeric F1–IF1 depended on the …

[PDF][PDF] Equilibrium between monomeric and dimeric mitochondrial F1^ inhibitor protein complexes

MT de Gömez-Puyou - FEBS Letters, 2001 - core.ac.uk
Mg-ATP particles from bovine heart mitochondria have more than 95% of their F1 in complex
with the inhibitor protein (IF1). The F1^ IF1 complex was solubilized and purified. The
question addressed was if this naturally occurring complex existed as monomers or dimers.
Size exclusion chromatography and electron microscopy showed that most of the purified
F1^ IF1 complex was a dimer of two F1^ IF1. As determined by the former method, the
relative concentrations of dimeric and monomeric F1^ IF1 depended on the concentration of …
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