Essential oil composition and fatty acid profile of Gypsophila tuberculosa Hub.-Mor. Gypsophila eriocalyx Boiss. were analyzed by GC-MS. Forty-one compounds were identified in the essential oil of G. tuberculosa that represent 81.4±0.5%(n= 3) of the oil. The major components of the oil were hexadecanoic acid (25.3±0.6%), hentriacontane (13.00±0.06%), dodecanoic acid (7.55±0.2%), tetradecanoic acid (6.53±0.1%) and pentacosane (3.06±0.07%). Sixty-one compounds were identified in the essential oil of G. eriocalyx that represent 70.32±1.53%(n= 3) of the oil. The major components of the oil were octacosane (6.83±0.2%), eicosanal (6.19±0.23%), triacontane (6.03±0.16%), heneicosane (5.78±0.2%), hexadecanoic acid (4.64±0.14%), tricosane (4.50±0.14%), hexahydrofarnesyl acetone (4.44±0.2%), heptacosane (3.40±0.12%), phytol (2.59±0.07%) and pentacosane (2.32±0.08%). Fourteen compounds were identified in the fatty acid of G. tuberculosa that represent 96.4±1.4%(n= 3) of the fatty acid. The major components of the fatty acid were (Z)-9-octadecenoic acid methyl ester (18: 1) 42.0±0.4%,(Z, Z)-9, 12-octadecadienoic acid methyl ester (18: 2) 19.6±0.3% and hexadecanoic acid methyl ester (16: 0) 17.7±0.4%(n= 3). Four compounds were identified in the fatty acid of G. eriocalyx that represent 71.8±0.9%(n= 3) of the fatty acid. The components of fatty acid were (Z)-9-octadecenoic acid methyl ester (18: 1) 36.0±0.2%, hexadecanoic acid methyl ester (16: 0) 25.2±0.5% and (Z, Z)-9, 12-octadecadienoic acid methyl ester (18: 2) 10.5±0.6 (n= 3).