Embryonic stem cells have the ability to remain undifferentiated and proliferate indefinitely in
vitro while maintaining the potential to differentiate into derivatives of all three embryonic
germ layers. These cells have, therefore, potential for in vitro differentiation studies, gene
function, and so on. The aim of this study was to produce a human embryonic stem cell line.
An inner cell mass of a human blastocyst was separated and cultured on mouse embryonic
fibroblasts in embryonic stem cell medium with related additives. The established line was …