The nature and magnitude of geographic genetic diversity among 11 genotypes of Pakistani Cupressussempervirens was studied through Sodium DodecylSulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Five populations representing different ecological conditions were sampled. A total of five loci (bands) were detected in the collected germplasm of cupresses sempervirens genotypes. Only one locus (locus-1) was found monomorphic while the remaining were polymorphic loci. Out of polymorphic loci, locus-5 showed a high degree of intra-specific locus variation (ISLV) ie 54.54% followed by Locus-3 which indicated 36.36% ISLVin the collected germplasm of C. sempervirence genotypes. Intra specie locus contribution towards genetic dis-agreement (LCTGD) was 80%. It was found that the genotypes of each zone occupied separate cluster in the dendrogram. PCA was carried out to complement the cluster analysis information. SDS-PAGE profiling based on two-way cluster plotting successfully resolved the genotypes on the basis of zone distribution. For better understanding of the genetic structure of C. sempervirencefurther analysis using dominant molecular markers are recommended.