ethanol production based on enzymatic hydrolysis was evaluated. MSW was subjected to a
thermal pretreatment (active hygienization) at 160° C from 5 to 50 min. The organic fiber
obtained after 30 min was used as substrate in a simultaneous saccharification and
fermentation (SSF) and fed-batch SSF process using cellulases and amylases. In a fed-
batch mode with 25%(w/w) substrate loading, final ethanol concentration of 30 g/L was …