To investigate human brain activities regarding EEG signal during psychophysiological activities in Salat (Muslim Prayer), several facts have been analyzed in this study. This research work investigated relaxed condition with eyes open and eyes closed and compared with 2 raqat (a single unit of Muslim prayer) Salat. Consequently, we have proposed that Salat provides a more relaxed state of mind than that of relaxing with either eye opened or closed. EEG data were acquired through the B-Alert system from several participants. The effects of EEG alpha band were determined using Welch's power spectral density method. Using student's t-distribution, the p-value was calculated to determine the difference between the alpha relative power of Salat and other relaxed states. During psychophysiological activities in Salat, a significant (p<;0.05) increase in alpha RP has been observed in the frontal and parietal regions than other two relaxed sessions. This result reflects the relaxed condition of body and soul which raises parasympathetic activity and lessens the sympathetic activity. Therefore, this proposed work concludes that Salat can support proper relaxation and reduce anxiety than the regular relaxed situations.