Vacuolization is an often observed characteristic of virus infection on cell lines during cell
based virus detection and isolation protocols and is commonly considered as a cytopathic
effect (CPE) resulting from virus infection. However, caution should be exercised when
vacuolization is used to indicate CPE as in addition to being induced by viruses,
vacuolization in cells can occur spontaneously, as a response to cellular damage, during
natural sequestration of materials and fluids, and fusion of many smaller vesicles formed by …