The present study was conducted for evaluation of Emblica officinalis (Amla) fruit for antibacterial activity and therapeutic potential in bovine sub-clinical mastitis. The fruit juices were extracted aseptically and the antibacterial activity against Staphylococcus aureus and Escherichia coli was studied revealing zone of inhibition of 19.7±0.05 mm and 18.81±0.07 mm by working dilution of E. officinalis (90/10 v/v) and the MIC and MBC of fruit juice of E. officinalis against S. aureus were observed to be 5.62% and 11.25% respectively and against E. coli were observed to be 22.5% and 45% respectively. In vivo study involved specific subclinical mastitic affected lactating cows divided into 2 groups (n= 6), Group 1 cows served as control administered with placebo treatment (wheat bran), cows of Group 2 were treated with E. officinalis deseeded fruits@ 250 gm total dose PO, divided in two parts morning and evening for 5 days. The elimination of infection in E. officinalis treatment vs. control was statistically non-significant (p> 0.05) with χ2 (01 df, N= 24)= 0.974, p= 0.324 however the therapy resulted significant decline on day 21 for California Mastitis Test score, Somatic Cell Count, electrical conductivity and pH of the milk. Therapy resulted significant increase in lactose on day 7 and day 21, however non-significant effect on fat, SNF and protein was observed. Therapy caused significant decline in percentage of mean neutrophil and significant improvement in the mean lymphocyte percentage on day 21.