The aim of the present study was the evaluation of hepatoprotective effect of Pistacia lentiscus fatty oil in rats intoxicated by carbon tetrachloride (CCL4). The study was conducted on 24 Wistar rats divided randomly to 4 groups. The first served as negative control (normal or CRL), the second intoxicated with CCl4 (CCl4 group), the 2 other groups were intoxicated by CCL4 and treated orally by 2ml/kg or 5ml/kg of PLFO (PLFO1 and PLFO2 groups). The results showed a significant elevation of ALT and AST enzymes in CCl4 group. PLFO2 recorded an aggravation of transaminases when compared to CCl4. However, PLFO1 has shown a significant decrease of AST with comparison to CCl4. We conclude that the use of PLFO to prevent hepatotoxicity has resulted in a partial activity by decreasing significantly ALT enzyme with the dose of 2ml/kg. However, the dose of 5ml/kg was found to aggravate the hepatic alterations by increasing significantly both of ALT and AST enzymes.