Evaluation of a rapid DNA extraction method to detect yeast cells by PCR in orange juice
M Ros-Chumillas, M Egea-Cortines, A Lopez-Gomez… - Food control, 2007 - Elsevier
Yeasts are the main causes of spoilage in pasteurized orange juice. Whereas detection by
plate counting techniques is too slow to allow appropriate intervention measures, PCR
reaction offers a rapid alternative, but it can be inhibited by components of food samples. We
developed a DNA extraction method directly from orange juice for rapid yeast detection by
PCR amplification of the rRNA internal transcribed spacers including the 5.8 S rRNA gene at
a detection limit of 103cfu/ml juice sample. We show that it is possible to reduce detection …
plate counting techniques is too slow to allow appropriate intervention measures, PCR
reaction offers a rapid alternative, but it can be inhibited by components of food samples. We
developed a DNA extraction method directly from orange juice for rapid yeast detection by
PCR amplification of the rRNA internal transcribed spacers including the 5.8 S rRNA gene at
a detection limit of 103cfu/ml juice sample. We show that it is possible to reduce detection …
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