Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
LM Rocha-Ramírez, B Hernández-Ochoa… - Microorganisms, 2020 - mdpi.com
Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the
host. However, it is unclear how probiotic bacteria regulate immune responses. The present
study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of
human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-
killed L. casei at a ratio (bacteria/MDM) of 50: 1, 100: 1, 250: 1, and 500: 1, and then
evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled …
host. However, it is unclear how probiotic bacteria regulate immune responses. The present
study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of
human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-
killed L. casei at a ratio (bacteria/MDM) of 50: 1, 100: 1, 250: 1, and 500: 1, and then
evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled …
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