been felt necessary for easy and effective estrus detection. This study was designed to
detect estrus in buffalo using a kit formulated in our laboratory based on pheromone
compound. Group I: Urine samples collected at estrus phase and group II: randomly
collected urine samples were subjected to the test using the kit. No colour developed (ie,
positive reaction) in estrus urine after adding the kit solution. By contrast, pale and/or dark …