Wheat germ oil posses various biological properties as an anticancer and antioxidant agent. Present study was undertaken to evaluate the radio protective ability of wheat germ (WG) oil against whole body irradiation rat. Wheat germ oil was given to rats by oral injection in a concentration of 1 ml/kg and 3 ml/kg body weight/dose for 3 successive days, last dose administered 24 h pre-irradiation exposure with an acute single dose level of 2 Gry delivered at a dose rate of 0.564 Gry/min at the time of experiment. With regard to cellular system, the results clearly indicated that pre-treatment with 3ml oil is more potent than 1ml and there are no significant differences between control group and groups that received oil only at either 1ml or 3ml in comparison to the control. Prior administration of WG oil to rats, significantly countered radiation induced biochemical disorder (liver enzymes and kidney function analysis, as well as, cholesterol level in the serum) and DNA damage (evaluated by DNA fragmentation assay and chromosomal aberration in bone marrow) in a dose dependent manner maximally at a concentration of 3 ml/kg. The results clearly indicated that wheat germ oil has significant potential to protect cellular system from radiation induced damage and ability to scavenge free radicals might be playing an important role in its radio protective manifestation without any toxicity