The present study investigated the reptilian fauna in Amasya Province, Turkey. Reptile species were identified from collections made during field studies or recorded in literature, with some new locality records obtained. Field studies were undertaken over two consecutive years (2016 and 2017). Two lacertid species, one skink species, two colubrid species and one viper species were officially recorded for the first time or their information was updated. In addition to species locality records, chorotypical and habitat selection were also assessed and the International Union for Conservation of Nature Red List of Threatened Species criteria included. Data on the distribution and locality information for each taxon is also provided. Our findings demonstrate that Amasya might be an ecotone zone between the Mediterranean, Caucasian, and European ecosystems. Although there are some concerns for the sustainable dynamics of reptilian fauna, relatively rich and different European nature information system habitat types provide basic survival conditions for reptilian fauna in the province.