The ancient pharmacological concept,“The Doctrine of Signatures”, is still evident among the tribal communities of Koraput district. This study explores the use of the doctrine of signatures prevalent among the Paraja and the Gadaba communities’ ethnic healers in the district. Various plant and animal features are conceived as indicative of medicinal properties owing to their resemblance with morphological features of biological organisms and parts affected by diseases. The research reports the traditional medicine use of a total of 29 numbers doctrine of signatures, out which twenty-four are plants and four are animal species and one is a natural substance, all total covering remedies for 20 different human sufferings.