Due to the contamination of freshwater resources, numerous techniques have been designed to address the challenges in achieving desalinated or freshwater over the past 50 years. Hydrate-based desalination (HBD) is one of the low-cost and efficient technologies receiving considerable interest of investigators in recent decades. Since R410a has the ability to form hydrates at mild conditions, in this research, three-stage desalination of synthesized seawater using R410a hydrate formation was carried out. The experiments were perormed in a stirred reactor at initial conditions of 11, 10, and 9 bar and 275.15 K. Moreover, cyclopentane was selected as a coformer for desalination experiments in the case of 9 bar. The desalination results showed that all of the above-mentioned conditions could remove 68–86.9% of dissolved ions and reduce the electrical conductivity to 75.3–83.2%. The experiments at an initial pressure of 9 bar and in the presence of cyclopentane had the best performance on ion removal efficiency and electrical conductivity, while the experiments at an initial pressure of 11 bar had the highest water recovery of 39.9%. The effect of the initial concentration of NaCl on desalination parameters illustrated that with an increase in the initial concentration, the removal efficiency enhances and water recovery declines.