The soluble polymers secreted by cell-suspension cultures of Nicotiana plumbaginifolia
contained 78% carbohydrate, 6% protein and 4% inorganic material. The extracellular
polysaccharides were separated into three fractions by anion-exchange chromatography
using a gradient of imidazole-HCl at pH 7 and the individual polysaccharides in each
fraction were then isolated by selective precipitation and enzymic treatment.
Monosaccharide and linkage compositions were determined for each polysaccharide after …