FMNH2-dependent monooxygenases initiate catabolism of sulfonamides in Microbacterium sp. strain BR1 subsisting on sulfonamide antibiotics
B Ricken, BA Kolvenbach, C Bergesch, D Benndorf… - Scientific reports, 2017 - nature.com
We report a cluster of genes encoding two monooxygenases (SadA and SadB) and one
FMN reductase (SadC) that enable Microbacterium sp. strain BR1 and other Actinomycetes
to inactivate sulfonamide antibiotics. Our results show that SadA and SadC are responsible
for the initial attack of sulfonamide molecules resulting in the release of 4-aminophenol. The
latter is further transformed into 1, 2, 4-trihydroxybenzene by SadB and SadC prior to
mineralization and concomitant production of biomass. As the degradation products lack …
FMN reductase (SadC) that enable Microbacterium sp. strain BR1 and other Actinomycetes
to inactivate sulfonamide antibiotics. Our results show that SadA and SadC are responsible
for the initial attack of sulfonamide molecules resulting in the release of 4-aminophenol. The
latter is further transformed into 1, 2, 4-trihydroxybenzene by SadB and SadC prior to
mineralization and concomitant production of biomass. As the degradation products lack …