Increased application of fertilizer to land pollutes the environment and sustainable productivity of land is hampered by the loss of Plant Growth Promoting Rhizobacteria. So an attempt was made to fabricate nano structured slow release fertilizer system associated with neem cake and Plant Growth Promoting Rhizobacteria. Nano structured fertilizer was prepared by ultra sonication and slow release fertilizer system was fabricated by a composite of neem cake, plant growth promoting rhizobacteria and nano NPK fertilizer. The influence of slow release fertilizer system on germination, mean germination time, seed vigour index, amylase, protease, total carbohydrates and total proteins of Vigna radiata was evaluated in pot study. Treatments for germination study included water control (T1), neem cake control (T2), NPK control (T3), phosphate solubilizing bacteria control (T4), potash solubilizing bacteria control (T5), nano NPK (T6), neem cake and nano NPK (T7), neem cake and phosphate solubilizing bacteria (T8), neem cake and potash solubilizing bacteria (T9) and slow release fertilizer system (T10). Size of nano fertilizer ranged from 64 to 73 nm. Germination was 85% in T1, 87% in T2, 82% in T3, 89% in T4, T5 and T6, 91% in T7, 86% in T8 and T9. 100% of germination was observed in slow release fertilizer system. Mean Germination Time in T1 was 42.5 and it was remarkably increased to 100 in T10. Seed vigour index was 423 in T1 and 1625 in T10. Slow release fertilizer system has accelerated the enzyme activity during germination and it was responsible for the observed increase in seed vigour index.