The expansion of automation techniques and increased risk of identity theft have led emphasis on the tremendous need of automated identification system. Due to the high recognition accuracy and robustness to changes in human physiology, retinal biometric identification system has drawn much attention in this research field. In this paper, we aim to propose an automatic fast and accurate retinal identification system for the multisample dataset. The proposed approach uses a hybrid segmentation technique to segment out both thick/thin vessels for effectively balancing the difference of wavelet response between thick/thin blood vessels. As a result, recognition accuracy is improved. A Principle Component Analysis-based feature processing approach is proposed for efficiently reducing the dimensionality of a large number of vessels features. It significantly reduces computation time and accelerates the matching process in the retinal identification system. The proposed technique is validated on DRIVE, STARE, VARIA, RIDB, HRF, Messidor, DIARETDB0, and a large multisample per subject database created by authors using the images provided by Dr. Chen (Shanghai Jiao Tong University Affiliated Sixth People Hospital). Experimental results demonstrated that the proposed approach outperforms other existing techniques. Segmentation achieves an overall accuracy of 99.65% with the recognition rate of 99.40% on all these databases.