… fatty acyl modification. In this Perspective, we focus the discussion on key issues pertaining
to fatty acylation of Wnts, … in the biochemistry of Wnt fatty acylation and provide a molecular …

… Another area of rapid expansion is fatty acylation of the secreted proteins hedgehog, Wnt …
membrane bound O-acyl transferases recognize their protein and fatty acyl CoA substrates is …

… chain is more than just a hydrophobic coating and appears to be critical for Wnt … in Wnt
monounsaturated fatty acylation and the mechanism by which this lipid moiety regulates Wnt …

… acylated, as well as the function of acylation. Our results indicate that another acylation with
a monounsaturated fatty … that Wnt-3a defective in acylation at this site does not exit the ER. …

… cis-trans isomerase, was affixed onto the WNT protein. We also note that WNT974 inhibited
trans fatty acylation of WNT protein, confirming the role of PORCN in this biochemical event (…

… To investigate the possible function of N-glycosylation or lipidation of Wnt proteins, we
generated mutant Wnt1 and Wnt3a polypeptides containing single or multiple amino acid …

… It is suggested that the fatty acyl adduct of Wnts restricts the Wnt signaling to adjacent cells.
The ER… of fatty acid metabolizing enzymes in the synthesis and modification of Wnt proteins is …

… of fatty acids as a critical contact residue. In the present article, we review our current
understanding of Wnt acylation and its functional role in Wnt signalling … of Wnt protein …

… Studies of PORCN activity relied on cell-based fatty acylation and signaling assays as … fatty
acylation of a Wnt substrate. The critical feature is the use of a double disulfide-bonded Wnt …

… Wnt proteins signal through frizzled (FZD) receptors to regulate physiological processes;
however, the structural basis for recognition of the Wnt unsaturated fatty acyl … with free fatty acids…