Frontiers in biocatalysis: profiling function across sequence space
A Rodríguez Benítez, ARH Narayan - 2019 - ACS Publications
2019•ACS Publications
… To profile how these “unknown” sequences fit within the family, the authors profiled the
activity of these enzymes against a panel of substrates and halide sources. This allowed the
researchers to identify trends in reactivity … In this study, 128 putative sequences from
across the FDH sequence space defined by the SSN were obtained as codon-optimized
genes. From this set, 87 proteins were successfully expressed in yields sufficient for
reactivity screening. By testing the reactivity of this enzyme panel with 12 substrates, the …
activity of these enzymes against a panel of substrates and halide sources. This allowed the
researchers to identify trends in reactivity … In this study, 128 putative sequences from
across the FDH sequence space defined by the SSN were obtained as codon-optimized
genes. From this set, 87 proteins were successfully expressed in yields sufficient for
reactivity screening. By testing the reactivity of this enzyme panel with 12 substrates, the …
High-throughput screening accelerates identification of biocatalysts for selective halogenation.
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