Functional display of complex cellulosomes on the yeast surface via adaptive assembly

SL Tsai, NA DaSilva, W Chen - ACS synthetic biology, 2013 - ACS Publications
… Inspired by this adaptive mechanism from nature, we report here the creation of a four…
cellulosome structure on the yeast surface by mimicking the adaptor scaffoldin-mediated assembly

Efficient yeast surface-display of novel complex synthetic cellulosomes

H Tang, J Wang, S Wang, Y Shen, D Petranovic… - Microbial Cell …, 2018 - Springer
… , were used for the self-assembly of cellulosome on the surface of yeast [13, 14]. Efforts have
also been made to improve the functional efficiency of yeast cellulosomes. For example, the …

Constructing a yeast to express the largest cellulosome complex on the cell surface

M Anandharaj, YJ Lin, RP Rani… - Proceedings of the …, 2020 - National Acad Sciences
cellulosome complex assembly on yeast cell surface using fluorescence microscopy. To form
a cellulosome complex … purified cellulosome confirmed the functionality of our cellulosome (…

Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production

C Dong, J Qiao, X Wang, W Sun, L Chen, S Li… - Biotechnology for …, 2020 - Springer
… ) microorganism by surface assembly of cellulosomes has been … In nature, the cellulosome
is a complicated multi-enzyme … the dead yeasts with surface-display cellulosomes have the …

The role of yeast-surface-display techniques in creating biocatalysts for consolidated bioprocessing

IDF Tabañag, IM Chu, YH Wei, SL Tsai - Catalysts, 2018 - mdpi.com
… , the term “yeast surface display” is adapted for convenience. … to cellulosomes in this article,
this yeast display strategy is … The resulting complex cellulosome assembly was then tested for …

A parts list for fungal cellulosomes revealed by comparative genomics

CH Haitjema, SP Gilmore, JK Henske… - Nature …, 2017 - nature.com
… , modular assembly mechanism and functional role of fungal … set of proteins critical to fungal
cellulosome assembly, including … —an independently evolved fungal complex that co-opted …

Advances in consolidated bioprocessing using synthetic cellulosomes

SL Tsai, Q Sun, W Chen - Current Opinion in Biotechnology, 2022 - Elsevier
… (b) Functional display of a trifunctional cellulosome using a four-member … complex cellulosome
via adaptive assembly have been reported. In one example, a yeast strain displaying an …

Cell-surface display of enzymes by the yeast Saccharomyces cerevisiae for synthetic biology

T Tanaka, A Kondo - FEMS Yeast Research, 2015 - search.proquest.com
… the cellulosome complex can be assembled onto the cell surface, … yeast consortium displaying
a functional minicellulosome. … surface via adaptive assembly. ACS Synth Biol 2013;18:14–…

Design and construction of synthetic cellulosome with three adaptor scaffoldins for cellulosic ethanol production from steam-exploded corn stover

S Tian, JL Du, ZS Bai, J He, XS Yang - Cellulose, 2019 - Springer
functional expression of intricate adaptive-assembling multienzyme … to direct the adaptive
assembly of the complex cellulosome. … yeast surface display of an intricate, active adaptive-…

Current challenges in designer cellulosome engineering

B Lamote, MJM da Fonseca, J Vanderstraeten… - Applied Microbiology …, 2023 - Springer
… that are assembled in vitro or are displayed on the yeast cell surface. … overall cellulosomal
structure and their natural functionality like … , protein purification and assembly of the complex. …