A series of substituted 2-aminothiazoles (AT-1 to AT-4) have been synthesized by reacting substituted acetophenones, iodine and thiourea under microwave irradiation as green chemistry approach. The reactions proceed selectively and within a couple of minutes giving high yields of the products. The compounds were characterized by spectral (UV-visible, IR, NMR and GC-MS) and thermal analyses. The TG curves of compounds were analyzed to calculate various kinetic parameters (n, E, Z, ΔS and G) using Coats–Redfern (CR), MacCallum-Tanner (MT) and Horowitz-Metzger (HM) method. The compounds were tested for antibacterial activity against B. subtilis and E. coli and antifungal activity against A. niger and C. albicans using serial dilution technique.