Gelatin speciation using real-time PCR and analysis of mass spectrometry-based proteomics datasets

B Jannat, K Ghorbani, H Shafieyan, S Kouchaki… - Food Control, 2018 - Elsevier
B Jannat, K Ghorbani, H Shafieyan, S Kouchaki, A Behfar, N Sadeghi, S Beyramysoltan
Food Control, 2018Elsevier
… In this study, Polymerase Chain Reaction (PCR) technique using species-specific
primers and also chemometrics analysis of mass spectral data were investigated to
differentiate bovine, porcine and fish source of gelatin in commercial pure gelatin and
gelatin-containing food and drug products. The specific PCR primer set was optimized
using real-time PCR approach to routinely determine the gelatin source. … Toward the goal
of developing an approach that would fulfill the aforementioned requirements, this study …
Highlights
  • Real-time PCR using species primers was implemented for gelatin source detection.
  • LC/MS data combined with chemometric methods to identify animal source of gelatin.
  • Distinguishing bovine, porcine and fish gelatin in food and drug products.
Elsevier
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