Fibroblasts from two Parkinson's disease (PD) patients carrying either the heterozygous
mutation c. 815G> A (Miro1 p. R272Q) or c. 1348C> T (Miro1 p. R450C) in the RHOT1 gene,
were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal
reprogramming, respectively. The corresponding isogenic gene-corrected lines have been
generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study
Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC …

Primary skin fibroblasts from two Parkinson's disease (PD) patients carrying distinct
heterozygous mutations in the RHOT1 gene encoding Miro1, namely c. 1290A> G (Miro1 p.
T351A) and c. 2067A> G (Miro1 p. T610A), were converted into induced pluripotent stem
cells (iPSCs) by episomal reprogramming. The corresponding isogenic gene-corrected lines
have been generated using CRISPR/Cas9 technology. Here, we provide a comprehensive
characterization and quality assurance of both isogenic pairs, which will be used to study …