Publisher Summary This chapter focuses largely on the more highly developed
saccharomyces cerevisiae system to summarize some basic features of yeast mitochondrial
genetics, describes the current methods for the delivery of DNA into the organelle, and
outlines the strategies to create directed mutations in mitochondrial genes and insert new
genes into mitochondrial DNA (mtDNA). It also presents the general strategy and protocol for
chlamydomonas mitochondrial transformation. The methods developed for manipulation of …