Genome oligopaint via local denaturation fluorescence in situ hybridization
Cas9 in complex with a programmable guide RNA targets specific double-stranded DNA for
cleavage. By harnessing Cas9 as a programmable loader of superhelicase to genomic
DNA, we report a physiological-temperature DNA fluorescence in situ hybridization (FISH)
method termed genome oligopaint via local denaturation (GOLD) FISH. Instead of global
denaturation as in conventional DNA FISH, loading a superhelicase at a Cas9-generated
nick allows for local DNA denaturation, reducing nonspecific binding of probes and avoiding …
cleavage. By harnessing Cas9 as a programmable loader of superhelicase to genomic
DNA, we report a physiological-temperature DNA fluorescence in situ hybridization (FISH)
method termed genome oligopaint via local denaturation (GOLD) FISH. Instead of global
denaturation as in conventional DNA FISH, loading a superhelicase at a Cas9-generated
nick allows for local DNA denaturation, reducing nonspecific binding of probes and avoiding …
Genome oligopaint via local denaturation fluorescence in situ hybridization (gold fish) and single-nucleotide variation detection
Cas9 in complex with a programmable guide RNA targets specific double-stranded DNA for
cleavage. By harnessing Cas9 as a programmable loader of superhelicase to genomic
DNA, we report a physiological-temperature DNA fluorescence in situ hybridization (FISH)
method termed genome oligopaint via local denaturation (GOLD) FISH. Instead of global
denaturation as in conventional DNA FISH, loading a superhelicase at a Cas9-generated
nick allows for local DNA denaturation, reducing nonspecific binding of probes and avoiding …
cleavage. By harnessing Cas9 as a programmable loader of superhelicase to genomic
DNA, we report a physiological-temperature DNA fluorescence in situ hybridization (FISH)
method termed genome oligopaint via local denaturation (GOLD) FISH. Instead of global
denaturation as in conventional DNA FISH, loading a superhelicase at a Cas9-generated
nick allows for local DNA denaturation, reducing nonspecific binding of probes and avoiding …
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