Granulocyte colony-stimulating factor (G-CSF) is used clinically to treat leukopenia and to
enforce hematopoietic stem cell (HSC) mobilization to the peripheral blood (PB). However,
G-CSF is also produced in response to infection, and excessive exposure reduces HSC
repopulation capacity. Previous work has shown that dormant HSCs contain all the long-
term repopulation potential in the bone marrow (BM), and that as HSCs accumulate a
divisional history, they progressively lose regenerative potential. As G-CSF treatment also …

Granulocyte colony stimulating factor (G-CSF) is used clinically to both treat neutropenias
and to enforce hematopoietic stem cell (HSC) mobilization to the peripheral blood. However,
G-CSF is also produced in response to infection, and excessive exposure is known to
reduce HSC repopulation capacity. Previous work from our lab demonstrated that dormant,
label-retaining hematopoietic stem/progenitor cells (HSPCs) contain most if not all the long-
term repopulation potential in the bone marrow (BM), and that as HSPCs accumulate a …